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I will do bioinformatics rnaseq and variant calling analysis
Bioinformatics and Data Analysis Specialist , Python , BLAST, ML
I'm a Bioinformatics student at NUST Islamabad with hands-on experience in sequence analysis, protein structure, phylogenetics, and Python-based data processing. I help researchers, students, and biot...
About this Gig
Got sequencing data and no time to build the pipeline yourself? I will take your raw reads and turn them into clean, interpretable results your PI or collaborators can actually use.
I am a Bioinformatics student at NUST Islamabad with hands-on experience in long-read sequencing pipelines (PacBio HiFi), variant calling with GATK and Clair3, benchmarking against GIAB truth sets, and genomic data analysis using Python and bash. I have worked in a cancer genomics research lab and understand what publication-quality output actually looks like.
What I can do for you:
Variant calling WGS alignment with minimap2/BWA, variant detection with GATK HaplotypeCaller or Clair3, VCF filtering and annotation, benchmarking with hap.py
RNA-seq differential expression count matrix DESeq2 or edgeR volcano plots, heatmaps, pathway enrichment
Metagenomics / 16S amplicon QIIME2 pipeline, alpha/beta diversity, PCoA plots, taxonomic bar charts
Genomic visualization IGV screenshots, coverage plots, publication-ready figures at 300 DPI
What you send me: FASTQ, BAM, VCF, count matrices, or a GEO / SRA accession number. I handle Illumina, PacBio, and Nanopore data.
What you get back: Clean output files, reprodu
Domain:
Other
Expertise:
Classification
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Clustering
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Predictive analysis
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Other
Programming language:
Python
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R
•
SQL
•
Colab
•
Java
Tools:
Jupyter Notebook
•
Other
Technology:
Python
•
R
•
Jupyter Notebook
•
Pandas
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RStudio
FAQ
I'm not sure what analysis I need. Can you help me figure it out?
A: Absolutely — just message me with your data type (FASTQ, BAM, count matrix, etc.) and your research question. I'll tell you exactly which package fits and what the output will look like before you order.
What files do I need to send you?
A: FASTQ, BAM, VCF, or count matrices work directly. If your data is on NCBI GEO or SRA, just send me the accession number — I'll download it myself. For large files, we can use Google Drive or a shared link.
Do you work with Illumina, PacBio, and Nanopore data?
A: Yes to all three. I have hands-on experience with PacBio HiFi long-read data specifically, and standard short-read Illumina pipelines for RNA-seq and WGS.
Will I be able to reproduce your results?
A: Yes. Every order includes fully documented bash/Python scripts with comments, so you or your lab can rerun the analysis at any time
Can I use your figures in my paper or thesis?
A: Yes — all figures are delivered at 300 DPI in PNG or PDF format, publication-ready for any journal.
How do I send you confidential or unpublished data?
I treat all client data as strictly confidential and do not share or retain it after delivery. If you need an NDA signed, message me before ordering.
