I will do rnaseq ngs analysis from raw reads to differential expression
Where Research Expertise Meets Academic Writing Excellence
About this Gig
I provide end-to-end RNA-seq and NGS analysis from raw sequencing reads to differential expression results and publication-ready visualizations.
What I deliver:
- Quality control & read trimming (FastQC, fastp)
- Alignment/quantification (Salmon)
- Differential expression analysis (DESeq2 in R)
- Visualizations: volcano plots, PCA plots, heatmaps
- Pathway/enrichment analysis (GO/KEGG) Premium package
- ML-based predictive modeling on expression data Premium package
Tools: Python, R, Salmon, DESeq2, tximport, ggplot2
Whether you need the full pipeline or help with just one stage for example, you already have a counts matrix and just need differential expression and plots I can start from wherever your data currently is.
FAQ
How many years of experience do you have in this field?
I have 5+ years of experience in this field. You won't regret it.
Can you take live discussion sessions?
You can do it anytime if you want to discuss it through your Fiverr inbox. But if you need a live discussion we can also do it.
What tools do you use?
Python and R, including Salmon, DESeq2, tximport, and standard visualization libraries.
Can I start midway if I already have alignment or counts done?
Yes — just send whatever stage your data is at and I'll pick up from there.
Will I get raw output or a full report?
You'll get processed results plus a report explaining the findings, with visualizations included at every tier.
What data formats do you accept?
FASTQ files, BAM files, or an existing counts matrix — I can start from any of these stages.

