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I will analyze ngs data and build reproducible bioinformatics pipelines
About this Gig
Need reliable analysis of your NGS sequencing data or a reproducible bioinformatics workflow?
I provide end-to-end bioinformatics and NGS data analysis, from raw sequencing data to clear, interpretable results. I can also develop reusable and containerized pipelines for research labs, scientists and biotech projects.
NGS analysis includes:
- Bulk RNA-seq and differential gene expression
- WGS, WES and targeted DNA sequencing
- Variant calling, filtering and annotation
- 16S rRNA and microbiome analysis
- FASTQ, BAM and VCF processing
- Quality control with FastQC and MultiQC
- Statistical analysis and data visualization
- Functional and pathway analysis
Reproducible pipeline development:
- Nextflow or Snakemake workflows
- Docker and Singularity/Apptainer support
- Conda/Bioconda environments
- Modular and configurable pipelines
- HPC/cloud-ready workflow design
- Testing and documentation
I work with Python, R and Bash and commonly used bioinformatics tools such as GATK, BWA, STAR, Salmon, DESeq2, Bioconductor and QIIME2.
You receive documented results, visualizations, code and a reproducible analysis tailored to your research question.
Please contact me before ordering to discuss your data, study design and
FAQ
What types of NGS data can you analyze?
I can analyze bulk RNA-seq, WGS, WES, targeted DNA sequencing and 16S rRNA/microbiome data. Depending on your project, I can work with FASTQ, BAM, VCF, count matrices and other standard bioinformatics formats.
Can you analyze raw FASTQ files from start to finish?
Yes. I can perform end-to-end NGS analysis including quality control, preprocessing, alignment or quantification, statistical analysis, visualization and downstream interpretation depending on the sequencing type and research question.
Do you provide RNA-seq differential expression analysis?
Yes. Bulk RNA-seq analysis can include QC, alignment or transcript quantification, count generation, differential expression with tools such as DESeq2, PCA, heatmaps, volcano plots and functional or pathway analysis.
Can you analyze WGS and WES data?
Yes. WGS, WES and targeted DNA-seq projects can include FASTQ quality control, alignment, BAM processing, variant calling, filtering and variant annotation. This service is intended for research and bioinformatics purposes, not medical diagnosis.
Can you build a reusable bioinformatics pipeline?
Yes. I can develop reproducible NGS pipelines using Nextflow or Snakemake with configurable inputs, modular workflow steps, documentation and dependency management so the analysis can be run again on future datasets.
Do your pipelines support Docker and Singularity/Apptainer?
Yes. Pipelines can be containerized using Docker and configured for Singularity/Apptainer, making them suitable for reproducible execution across local systems, servers and compatible HPC environments.
What results will I receive?
Depending on your project, deliverables may include QC reports, processed data, statistical results, tables, figures, annotated variants, differential expression results, pathway analysis, scripts, documentation and pipeline source code.
