I will do restriction digestion, rt pcr, western blotting

I will give you the optimized parameters for the Polymerase Chain Reaction (PCR), moreover, I will give you recommendations on the selection of plasmid for your specific application. I will give you possible options for the restriction digestion enzymes for a particular gene-plasmid system cloning. a schematic diagram of your personalized recombinant DNA molecule. An extensive review of the engineered protein and on the protein purifcation.