I will do bioinformatics rna seq dna seq analysis and variant calling
LLM Developer
About this Gig
Need reliable, publication-ready bioinformatics analysis? You're in the right place.
I provide end-to-end NGS analysis for RNA-Seq and DNA-Seq, including full variant calling and annotation, following GATK Best Practices and current gold-standard tools.
RNA-Seq: quality control, trimming, alignment (STAR / HISAT2 / Salmon), quantification, differential expression (DESeq2 / edgeR), and functional enrichment (GO / KEGG / GSEA) with PCA, volcano plots, and heatmaps.
DNA-Seq & Variant Analysis: alignment (BWA-MEM), duplicate marking, BQSR, variant calling (GATK HaplotypeCaller / Mutect2 / DeepVariant / bcftools), plus annotation (VEP / ANNOVAR / SnpEff) and filtered, prioritized variant tables.
You receive clean, reproducible results: processed files, annotated tables, high-resolution figures, and a clear methods summary you can drop straight into a paper or report.
I work with human, model, and non-model organisms across germline and somatic studies.
New project or not sure what you need? Message me first and I'll map the exact pipeline and quote for your data.
My Portfolio
FAQ
Do I need to provide the raw data?
Yes — FASTQ is ideal, but I can start from BAM/VCF too. I can also pull public data from SRA/GEO if you give accession IDs.
Which organisms do you support?
Human, common model organisms, and non-model species (I build a custom reference when no standard annotation exists).
Can you do somatic / tumor-normal variant calling?
Yes — Mutect2-based somatic pipelines alongside standard germline HaplotypeCaller workflows.
Will the results be reproducible?
Every order includes documented steps; add the pipeline extra for fully scripted Nextflow/Snakemake reproducibility.
Is my data kept confidential?
Yes. Data is used only for your analysis and deleted on request after delivery.

