I will design and review your flow cytometry panel, gating and paper figures

Taiwan

I speak Chinese, English

PhD immunologist, flow cytometry panel, gating and figure review

I'm a Ph.D. immunologist working in an Academia Sinica research environment, focused on bone marrow and hematopoietic stem/progenitor cell (HSPC) biology. Day to day I design and run multicolor and sp...
About this Gig

Stuck on a flow panel, a gating strategy that won't hold up, or a reviewer asking about your FACS plots? AI can draw gates; it can't tell you whether they're biologically right.


I'm a Ph.D. immunologist working in an Academia Sinica research environment on immunology and hematology. Most of my work is bone marrow and hematopoietic stem/progenitor cell (HSPC) flow, including rare populations and spectral panels.


What I do:

- Panel design: marker and fluorochrome choice, spillover and spread, controls (FMO, single stains, viability)

- Gating strategy and troubleshooting: messy compensation or unmixing, dim markers, doublets, rare-event gates

- Pre-submission review: I check your flow figures and gating against what reviewers usually question, and give you clear, prioritized fixes

- Reviewer response: help drafting the flow-related answers and any new figures


Add-on: hands-on FlowJo analysis of your FCS files, publication-ready plots, and Python statistics. scRNA-seq comparison is possible on request.


Please message me before ordering with your species, tissue, cytometer and goal, and I'll confirm the scope. Your data stays confidential.

Technology:

Jupyter Notebook

Analysis Type:

Quantitative Analysis

•

Statistical Analysis

Expertise:

Algorithms

•

Cluster analysis

Programming language:

Python

Tools:

RStudio

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