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I will perform bacterial wgs and comparative genomics analysis
Cofounder The Freelance Bubble
As Co-Founder of The Freelance Bubble, I work with a multidisciplinary team of 23+ PhD scholars delivering high-quality research and technical support. I am a biotechnologist turned bioinformatician s...
Level 2
Has met high performance criteria and has a proven track record for meeting client expectations.
About this Gig
At The Freelance Bubble, we provide professional bacterial whole-genome sequencing (WGS) and comparative genomics analysis for researchers, laboratories, and academic teams working with microbial genomes.
Our services can include:
- Raw FASTQ quality control and trimming
- De novo genome assembly
- Assembly quality assessment
- Genome annotation
- Species confirmation and MLST
- Antimicrobial resistance gene profiling
- Virulence factor screening
- Plasmid detection
- ANI and genome similarity analysis
- SNP-based comparative analysis
- Core-genome and phylogenetic analysis
- Pan-genome analysis
- Core, accessory, and unique gene profiling
- Comparative genomics across multiple isolates
- Publication-ready figures and tables
We use established bioinformatics tools such as FastQC, fastp, SPAdes, QUAST, Bakta/Prokka, MLST, AMRFinderPlus, Abricate, FastANI, Snippy, IQ-TREE, Panaroo/Roary, and related workflows where appropriate.
Deliverables can include annotated genomes, phylogenetic trees, comparative plots, AMR/virulence summaries, scripts, and interpretation.
Please message us before ordering for large isolate collections, raw FASTQ datasets, pan-genome projects, or custom comparative genomics workflows.
My Portfolio
FAQ
What type of bacterial sequencing data can you analyze?
I can work with raw FASTQ reads, assembled genomes, annotated genomes, or publicly available bacterial genome datasets.
Can you analyze raw FASTQ files?
Yes. Raw-read processing can include quality control, trimming, de novo assembly, assembly assessment, and downstream genomic analysis.
What analyses are included in bacterial WGS?
Depending on the package, analysis can include assembly, annotation, MLST, AMR genes, virulence factors, plasmids, ANI, SNP analysis, phylogeny, and comparative genomics.
Can you perform pan-genome analysis?
Yes. Pan-genome analysis can include core, accessory, and unique gene profiling, clustering, comparative visualization, and interpretation.
Can you identify antimicrobial resistance genes?
Yes. AMR profiling can be performed using established databases and tools such as AMRFinderPlus, CARD-compatible workflows, or related resources where appropriate
Can you perform phylogenetic analysis?
Yes. I can perform SNP-based or core-genome phylogenetic analysis and provide publication-ready phylogenetic trees.
Can you compare multiple bacterial isolates or strains?
Yes. Comparative genomics can include ANI, gene-content comparison, SNP analysis, pan-genomics, AMR/virulence comparison, and phylogenetic relationships.
Will I receive publication-ready figures and tables?
Yes. Depending on the selected package, deliverables can include phylogenetic trees, comparative plots, heatmaps, genomic summaries, and manuscript-ready tables.
Will you provide scripts and analysis files?
Yes. Reproducible scripts, intermediate outputs, and relevant analysis files can be provided where applicable.
Should I contact you before ordering?
Yes. Please message us first for large isolate collections, raw sequencing datasets, pan-genome projects, unusual bacterial species, or custom pipelines so we can confirm scope and turnaround time.
